Composite

Part:BBa_K2207016:Design

Designed by: Junming Qian   Group: iGEM17_ZJU-China   (2017-10-26)


phlAB enzyme


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5143
    Illegal SpeI site found at 3321
    Illegal SpeI site found at 5309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5143
    Illegal SpeI site found at 3321
    Illegal SpeI site found at 5309
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5143
    Illegal XhoI site found at 2678
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5143
    Illegal SpeI site found at 3321
    Illegal SpeI site found at 5309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5143
    Illegal SpeI site found at 3321
    Illegal SpeI site found at 5309
    Illegal NgoMIV site found at 2978
    Illegal NgoMIV site found at 3265
    Illegal NgoMIV site found at 3832
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1516
    Illegal SapI site found at 414
    Illegal SapI.rc site found at 1363


Design Notes

We constructed a expression system according to the mechanism of M2S intergration technique, so that we can intergrate multiple genes into the chromosome.


Source

We successfully cloned the phlAB via colony PCR from Pseudomonas fluorescens 2P24, which is a gift from Prof. Liqun Zhang of China Agriculture University.

References

Li S, Ding W, Zhang X, et al. Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in Saccharomyces cerevisiae[J]. J Immunoassay Immunochem, 2016, 9(1):232.